LOCUS       sequence-101663-         497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 
            for a translocation-based green fluorescent caspase-3 biosensor, 
            used together with plasmid 2 (BNLS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GANLS-DEVD-BNES
FEATURES             Location/Qualifiers
     source          1..497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(230..248)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtgccgc caccatggcc tcgagaatgg tgagcaagag cgaggaggtc
      121 atcaaagagt tcatgcgctt caaggtgcgc ttggagggct ccatgaacgg ccacgagttc
      181 gagatcgagg gcgagggcga gggccgccct tacgagggca cccagaccgc caagctgaag
      241 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccct gatcatgtac
      301 ggctccaaga tgtacgtgaa gcaccccgcc gacgtccccg attacatgaa gctgtccttc
      361 cccgagggct tcaagtggga gcgcgtgatg cacttcgagg acggcggtct ggtgaccgcg
      421 acacaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
      481 aacttccccc ccgacgg
//
