LOCUS       sequence-101664-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 
            for a translocation-based green fluorescent caspase-3 biosensor, 
            used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: BNLS
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(236..254)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     504..523
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     698..721
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtgccgc caccatggcc tcgagagcct cctccgagga caacaacatg
      121 gccaccatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
      181 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      241 ctgaaggtga ccaagggcgg ccccctgccc tttgcctggg acatcctgtc cccccagatc
      301 atgtacggct ccaaagcgta cgtgaggcac cccgccgaca tccccgatta caagaagctg
      361 cccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaagacgg cggtctggtg
      421 accgttaccc aggactcctc cctgcaggac ggcacgctga tctgcaaggt gaagattcgc
      481 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      541 tccaccgaga tgctgtaccc caaggacggc gtgctgaagg gccatagcta tcaggccctg
      601 aagctgaagg acggcggcca ctacctggtg gagttcgaga ccatctacat ggccaagaag
      661 cccgtgcaac tgcccggcga ttactgtgtg gacaccaagc tggacatcac ctcccacaac
      721 gaggactaca ccatcgtgga acagtacggg cgctccgagg gccgccaccg tctgggcatg
      781 gacgagcggt acaagggaac ggcgg
//
