LOCUS       sequence-101668-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose-inducible repeat-only E. coli CRISPR array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Luo ML, Mullis AS, Leenay RT, Beisel CL
  TITLE     Repurposing endogenous type I CRISPR-Cas systems for programmable 
            gene repression.
  JOURNAL   Nucleic Acids Res. 2014 Oct 17. pii: gku971.
  PUBMED    25326321
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Oct 17. pii: gku971."
COMMENT     Sequence Label: pcrRNA.ind
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..125
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(275..559)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(333..352)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
       61 tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta
      121 tcaggctgaa aatcttctct catccgccaa aacagccaag cttaaaaaaa accccgccct
      181 gtcaggggcg gggttttttt ttcggtttat ccccgctggc gcggggaact cgaggtggta
      241 ccgagctcga attcgctagc ccaaaaaaac gggtatggag aaacagtaga gagttgcgat
      301 aaaaagcgtc aggtaggatc cgctaatctt atggataaaa atgctatggc atagcaaagt
      361 gtgacgccgt gcaaataatc aatgtggact tttctgccgt gattatagac acttttgtta
      421 cgcgtttttg tcatggcttt ggtcccgctt tgttacagaa tgcttttaat aagcggggtt
      481 accggtttgg ttagcgagaa gagccagtaa aagacgcagt gacggcaatg tctgatgcaa
      541 tatggacaat tggtttcttc tctgaatggc gggagtatga aaagtatggc tgaagcgcaa
      601 aatgatcccc tgctgccggg atactcgttt aatgcccatc tggtggcggg tttaacgccg
      661 attgaggcca acggttatct cgattttttt atcgaccgac cgctgggaat gaaaggttat
      721 attctcaatc tcaccattcg cggtca
//
