LOCUS       sequence-10167-e        4416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTER_MDC1 (422-2)
FEATURES             Location/Qualifiers
     source          1..4416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     171..190
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    236..254
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(427..444)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    433..657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      703..1131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(790..809)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1000..1021
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1140..1160)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1145..1474
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1325..1460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1387..1406
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1522..1569
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1588..1962
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2092..2213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2129..2148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2183..2202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2262..2278)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2262..2278)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2275..2297)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2286..2302
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2310..2316,2317..2334,2335..2340))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2355..2376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2493..2510)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2664..3249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2744..2763)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3420..4211,4212..4280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4043..4062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4281..4385)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccccc gggctgcagg aattcgaacg ctgacgtcat caacccgctc
       61 caaggaatcg cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca
      121 ggaagatggc tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg
      181 ttctgggaaa tcaccataaa cgtgaaatgt ctttggattt gggaatctta taagttccct
      241 atcagtgata gagatccccc aacatgcaga gattgaaatt caagagattt caatctctgc
      301 atgttgtttt tggaaaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
      361 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
      421 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
      481 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
      541 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
      601 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
      661 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
      721 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      781 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      841 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      901 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      961 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     1021 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     1081 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     1141 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1201 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     1321 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1381 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     1441 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     1501 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     1561 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     1621 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     1681 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     1741 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     1801 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     1861 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     1921 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     1981 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     2041 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     2101 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     2161 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     2221 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     2281 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2341 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2401 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2461 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2521 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2581 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2641 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2761 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2821 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2881 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3001 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3061 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3121 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3181 caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3301 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3361 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3661 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4021 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4261 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4321 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4381 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
