LOCUS       sequence-101673-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FPX biosensor, a new class of genetically encoded fluorescent 
            biosensor based on reversible exchange of the heterodimeric partners
            of green and red dimerization dependent fluorescent proteins 
            (ddFPs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: single polypeptide FPX biosensor for caspase-3
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(229..247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     497..516
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg ccaccatggt gagcaagagc gaggaggtca
      121 tcaaagagtt catgcgcttc aaggtgcgct tggagggctc catgaacggc cacgagttcg
      181 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      241 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag atcatgtacg
      301 gctccaaggc gtacgtgaag caccccgccg acgtccccga ttacatgaag ctgtccttcc
      361 ccgagggctt caagtgggag cgcgtgatgc acttcgagga cggcggtctg gtgaccgtga
      421 ctcaggacac ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
      481 acttcccccc cgacggcccc gtaatgcaga agaagacctt gggctgggat tatgccaccg
      541 agcgcctgta ccccgaagac ggcgtgctga agggcgagct tctggggcgc ctgaagctga
      601 aggacggcgg cctcaacctg gtggagttca agaccatcta catggccaag aagcccgtgc
      661 aactgcccgg ctactacttc gtggacacca agctgg
//
