LOCUS       sequence-101678-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 
            for a translocation-based red fluorescent caspase-8 biosensor, used 
            together with plasmid 2 (BNLS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: RANLS-IETD-BNES
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(230..248)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     498..517
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtgccgc caccatggcc tcgagaatgg tgagcaagag cgaggaggtc
      121 atcaaagagt tcatgcgctt caaggtgcgc ttggagggct ccatgaacgg ccacgagttc
      181 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      241 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gatcatgtac
      301 ggctccaagg cgtacgtgaa gcaccccgcc gacgtccccg attacatgaa gctgtccttc
      361 cccgagggct tcaagtggga gcgcgtgatg cacttcgagg acggcggtct ggtgaccgtg
      421 actcaggaca cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
      481 aacttccccc ccgacggccc cgtaatgcag aagaagacct tgggctggga ttatgccacc
      541 gagcgcctgt accccgaaga cggcgtgctg aagggcgagc ttctggggcg cctgaagctg
      601 aaggacggcg gcctcaacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
      661 caactgcccg gctactactt cgtggacacc aagctgggca tcacctccca caacgaggac
      721 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctggg catggacgag
      781 ctgtacaagg gaacggcggg aagcgatcca a
//
