LOCUS       sequence-101687-         443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ddGFP A-NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GA-NES
FEATURES             Location/Qualifiers
     source          1..443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(214..232)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtg
       61 ccgccaccat ggcctcgaga atggtgagca agagcgagga ggtcatcaaa gagttcatgc
      121 gcttcaaggt gcgcttggag ggctccatga acggccacga gttcgagatc gagggcgagg
      181 gcgagggccg cccttacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
      241 ccctgccctt cgcctgggac atcctgtccc ccctgatcat gtacggctcc aagatgtacg
      301 tgaagcaccc cgccgacgtc cccgattaca tgaagctgtc cttccccgag ggcttcaagt
      361 gggagcgcgt gatgcacttc gaggacggcg gtctggtgac cgcgacacag gactcctccc
      421 tgcaggacgg cacgctgatc tac
//
