LOCUS       sequence-101692-         401 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  green ddFP based caspase-3 biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GA-DEVD-GB-NES
FEATURES             Location/Qualifiers
     source          1..401
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(229..247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg ccaccatggc gagcaagagc gaggaggtca
      121 tcaaagagtt catgcgcttc aaggtgcgct tggagggctc catgaacggc cacgagttcg
      181 agatcgaggg cgagggcgag ggccgccctt acgagggcac ccagaccgcc aagctgaagg
      241 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtcccccctg atcatgtacg
      301 gctccaagat gtacgtgaag caccccgccg acgtccccga ttacatgaag ctgtccttcc
      361 ccgagggctt caagtgggag cgcgtgatgc acttcgagga c
//
