LOCUS       sequence-101695-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 
            for a translocation-based green fluorescent caspase-3 biosensor, 
            used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: BNLS
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..42)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(46..66)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(159..182)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(357..376)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     626..644
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      792..801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(825..844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt
       61 ttttggatcg cttcccgccg ttcccttgta ccgctcgtcc atgcccagac ggtggcggcc
      121 ctcggagcgc ccgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      181 cttggtgtcc acacagtaat cgccgggcag ttgcacgggc ttcttggcca tgtagatggt
      241 ctcgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctgat agctatggcc
      301 cttcagcacg ccgtccttgg ggtacagcat ctcggtggag gcctcccagc ccatggtctt
      361 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgaatcttca ccttgcagat
      421 cagcgtgccg tcctgcaggg aggagtcctg ggtaacggtc accagaccgc cgtcttcgaa
      481 gttcatcacg cgctcccact tgaagccctc ggggaagggc agcttcttgt aatcggggat
      541 gtcggcgggg tgcctcacgt acgctttgga gccgtacatg atctgggggg acaggatgtc
      601 ccaggcaaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc
      661 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc
      721 catgcgcacc ttgaagcgca tgaactcttt gatggtggcc atgttgttgt cctcggagga
      781 ggctctcgag gccatggtgg cggcacgcta gccagcttgg gtctccctat agtgagtcgt
      841 attaatttcg ataagccagt aa
//
