LOCUS       sequence-101702-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses one ddFP in mammalian cells, the second plasmid for a 
            green to red colour switch-based fluorescent caspase-3 biosensor, 
            used together with plasmid GANES-DEVD-BNLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: RANLS
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..42)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(46..66)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(357..376)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     626..644
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      786..795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(819..838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(820..838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt
       61 ttttggatcg cttcccgccg ttcccttgta cagctcgtcc atgcccaggt ggtggcggcc
      121 ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgcccag
      181 cttggtgtcc acgaagtagt agccgggcag ttgcacgggc ttcttggcca tgtagatggt
      241 cttgaactcc accaggttga ggccgccgtc cttcagcttc aggcgcccca gaagctcgcc
      301 cttcagcacg ccgtcttcgg ggtacaggcg ctcggtggca taatcccagc ccaaggtctt
      361 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgcatcttca ccttgtagat
      421 cagcgtgccg tcctgcaggg aggtgtcctg agtcacggtc accagaccgc cgtcctcgaa
      481 gtgcatcacg cgctcccact tgaagccctc ggggaaggac agcttcatgt aatcggggac
      541 gtcggcgggg tgcttcacgt acgccttgga gccgtacatg atctgggggg acaggatgtc
      601 ccaggcgaag ggcagggggc cgcccttggt caccttcagc ttggcggtct gggtgccctc
      661 gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg tggccgttca tggagccctc
      721 caagcgcacc ttgaagcgca tgaactcttt gatgacctcc tcgctcttgc tcaccattct
      781 cgaggccatg gtggcggcac gctagccagc ttgggtctcc ctatagtgag tcgtattaat
      841 ttcgataagc cagtaagcag tgggttctct agttagccag a
//
