LOCUS       sequence-101705-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses one ddFP in mammalian cells, the second plasmid for a red 
            to green colour switch-based fluorescent caspase-3 biosensor, used 
            together with plasmid NESRA-DEVD-BNLS. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GANLS
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..42)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(46..66)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(156..179)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(354..373)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     623..641
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      783..792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(816..835)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(817..835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttctcttc ttttttggat ctacctttct cttctttttt ggatctacct ttctcttctt
       61 ttttggatcg cttcccgccg ttccgctacc tgtaccatgc cccaggtggt ggcggccctc
      121 ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      181 ggtgtccacg aagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt
      241 ggactccacc aggttgaggc cgccgtcctt cagcttcagg cgccccagga gctcgccctt
      301 cagcacgcct tcttcggggt acaggcgctc ggtggcataa tcccagccca aggtcttctt
      361 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag
      421 cgtgccgtcc tgcagggagg agtcctgtgt cgcggtcacc agaccgccgt cctcgaagtg
      481 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcatgtaat cggggacgtc
      541 ggcggggtgc ttcacgtaca tcttggagcc gtacatgatc aggggggaca ggatgtccca
      601 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      661 agggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccaa
      721 gcgcaccttg aagcgcatga actctttgat gacctcctcg ctcttgctca ccattctcga
      781 ggccatggtg gcggcacgct agccagcttg ggtctcccta tagtgagtcg tattaatttc
//
