LOCUS       sequence-101706-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FPX biosensor, a new class of genetically encoded fluorescent 
            biosensor based on reversible exchange of the heterodimeric partners
            of green and red dimerization dependent fluorescent proteins 
            (ddFPs)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: single polypeptide FPX biosensor for caspase-3
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(122..145)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(320..339)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     589..607
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gactcgagct agctcccgat atcaagacct gctaatttca aggctaaggt gctgccggtg
       61 ccatgcccca ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgtagtcc
      121 tcgttgtggg aggtgatgtc cagcttggtg tccacgaagt agtagccggg cagttgcacg
      181 ggcttcttgg ccatgtagat ggtcttggac tccaccaggt tgaggccgcc gtccttcagc
      241 ttcaggcgcc ccaggagctc gcccttcagc acgccttctt cggggtacag gcgctcggtg
      301 gcataatccc agcccaaggt cttcttctgc attacggggc cgtcgggggg gaagttggtg
      361 ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc ctgtgtcgcg
      421 gtcaccagac cgccgtcctc gaagtgcatc acgcgctccc acttgaagcc ctcggggaag
      481 gacagcttca tgtaatcggg gacgtcggcg gggtgcttca cgtacatctt ggagccgtac
      541 atgatcaggg gggacaggat gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc
      601 agcttggcgg tctgggtgcc ctcgtaaggg cggccctcgc cctcgccctc gatctcgaac
      661 tcgtggccgt tcatggagcc ctccaagcgc accttgaagc gcatgaactc tttgatgacg
      721 gctccatcca cctcatcgcc ggaggcggat ccggagctgc cgctgccggt gctgcccttg
      781 taccgctcgt ccatgcccag acggtggcgg ccctcggagc gcccgtactg ttccacgatg
      841 gtgtagtcct cgttgtgg
//
