LOCUS       sequence-101707-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FPX biosensor for caspase-8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: single polypeptide FPX biosensor for caspase-8
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..144)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(319..338)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     588..606
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 actcgagcta gctcccgata tcaagacctg ctaatttcaa ggctaaggtg ctgccggtgc
       61 catgccccag gtggtggcgg ccctcggagc gctcgtactg ttccacgatg gtgtagtcct
      121 cgttgtggga ggtgatgtcc agcttggtgt ccacgaagta gtagccgggc agttgcacgg
      181 gcttcttggc catgtagatg gtcttggact ccaccaggtt gaggccgccg tccttcagct
      241 tcaggcgccc caggagctcg cccttcagca cgccttcttc ggggtacagg cgctcggtgg
      301 cataatccca gcccaaggtc ttcttctgca ttacggggcc gtcggggggg aagttggtgc
      361 cgcgcatctt caccttgtag atcagcgtgc cgtcctgcag ggaggagtcc tgtgtcgcgg
      421 tcaccagacc gccgtcctcg aagtgcatca cgcgctccca cttgaagccc tcggggaagg
      481 acagcttcat gtaatcgggg acgtcggcgg ggtgcttcac gtacatcttg gagccgtaca
      541 tgatcagggg ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca
      601 gcttggcggt ctgggtgccc tcgtaagggc ggccctcgcc ctcgccctcg atctcgaact
      661 cgtggccgtt catggagccc tccaagcgca ccttgaagcg catgaactct ttgatgacgg
      721 taccacccac cgcaccgccg gaggcggatc cggagctgcc gctgccggtg ctgcccttgt
      781 accgctcgtc catgcccaga cggtggcggc cctcggagcg cccgtactgt tccacgatgg
      841 tgtagtcctc gttgtg
//
