LOCUS       sequence-101710-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FPX biosensor for caspase-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: single polypeptide FPX biosensor for caspase-1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(86..109)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(284..303)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     553..571
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gactcgagct aggtgctgcc ggtgccatgc cccaggtggt ggcggccctc ggagcgctcg
       61 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg
      121 aagtagtagc cgggcagttg cacgggcttc ttggccatgt agatggtctt ggactccacc
      181 aggttgaggc cgccgtcctt cagcttcagg cgccccagga gctcgccctt cagcacgcct
      241 tcttcggggt acaggcgctc ggtggcataa tcccagccca aggtcttctt ctgcattacg
      301 gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct tgtagatcag cgtgccgtcc
      361 tgcagggagg agtcctgtgt cgcggtcacc agaccgccgt cctcgaagtg catcacgcgc
      421 tcccacttga agccctcggg gaaggacagc ttcatgtaat cggggacgtc ggcggggtgc
      481 ttcacgtaca tcttggagcc gtacatgatc aggggggaca ggatgtccca ggcgaagggc
      541 agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta agggcggccc
      601 tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg agccctccaa gcgcaccttg
      661 aagcgcatga actctttgat gacctcctcg ctcttgctca ccatatccgc cacataaccc
      721 catccatccg ccacatagcc ccatccatcc gccacatagc cggatccgga gctgccgctg
      781 ccggtgctgc ccttgtaccg ctcgtccatg cccagacggt ggcggccctc ggagcg
//
