LOCUS       sequence-101713-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HTLV-1 based transfer vector encoding gfp-turbo reporter gene 
            interrupted by intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, 
            Nikolaitchik O, Hu WS, Mazurov D
  TITLE     Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 
            Replication-Dependent Vectors via Optimization of Reporter Gene 
            Reconstitution and Modification with Intronic Short Hairpin RNA.
  JOURNAL   J Virol. 2015 Oct 15;89(20):10591-601. doi: 10.1128/JVI.01940-15. 
            Epub 2015 Aug 12.
  PUBMED    26269177
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01940-15"; journalName: "J Virol"; date: "2015-10-15- 
            15"; volume: "89"; issue: "20"; pages: "10591-601"
COMMENT     Sequence Label: pCRU5-inGFPt
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           458..578
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           606..620
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gcgtctagtc gacggatatc cacgcgttat tcttcaccgg catctgcatc cggggtcttg
       61 aaggcgtgct ggtactccac gatgcccagc tcggtgttgc tgtgatcctc ctccacgcgg
      121 cggaaggcga acatggggcc cccgttctgc aggatgctgg ggtggatggc gctcttgaag
      181 tgcatgtggc tgtccaccac ggagctgtag tagccgccgt cgcgcaggct gaaggtgcgg
      241 gtgaagctgc catccaggtg agtccaggag atgtttcagc actgttgcct ttagtctcga
      301 ggcaacttag acaactgagt attgatctga gcacagcagg gtgtgagctg tttgaagata
      361 ctggggttgg gggtgaagaa actgcagagg actaactggg ctgagaccca gtggcaatgt
      421 tttagggcct aaggaatgcc tctgaaaatc tagatgtttg aatgaggctt cagtacttta
      481 cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcttc aggttaaccc
      541 aacagaaggc tcgagaaggt atatgctgtt gacagtgagc gaaggacaag tatggtcatt
      601 aaatagtgaa gccacagatg tatttaatga ccatacttgt cctgtgccta ctgcctcgga
      661 tcccaagggg ctactttagg agcaattatc ttgtttacta aaactgaata ccttgctatc
      721 tctttgatac atttttacaa agctgaatta aaatggtata aattaaatca cttctagatg
      781 gacaactttg actttga
//
