LOCUS       sequence-101717-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 transfer vector that encodes gfp-turbo reporter gene 
            interrupted by intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, 
            Nikolaitchik O, Hu WS, Mazurov D
  TITLE     Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 
            Replication-Dependent Vectors via Optimization of Reporter Gene 
            Reconstitution and Modification with Intronic Short Hairpin RNA.
  JOURNAL   J Virol. 2015 Oct 15;89(20):10591-601. doi: 10.1128/JVI.01940-15. 
            Epub 2015 Aug 12.
  PUBMED    26269177
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01940-15"; journalName: "J Virol"; date: "2015-10-15- 
            15"; volume: "89"; issue: "20"; pages: "10591-601"
COMMENT     Sequence Label: pUCHR-inGFPt
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           467..587
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           615..629
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 atagcggtcg gccgctttac ttgtacatcc acgcgttatt cttcaccggc atctgcatcc
       61 ggggtcttga aggcgtgctg gtactccacg atgcccagct cggtgttgct gtgatcctcc
      121 tccacgcggc ggaaggcgaa catggggccc ccgttctgca ggatgctggg gtggatggcg
      181 ctcttgaagt gcatgtggct gtccaccacg gagctgtagt agccgccgtc gcgcaggctg
      241 aaggtgcggg tgaagctgcc atccaggtga gtccaggaga tgtttcagca ctgttgcctt
      301 tagtctcgag gcaacttaga caactgagta ttgatctgag cacagcaggg tgtgagctgt
      361 ttgaagatac tggggttggg ggtgaagaaa ctgcagagga ctaactgggc tgagacccag
      421 tggcaatgtt ttagggccta aggaatgcct ctgaaaatct agatgtttga atgaggcttc
      481 agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca
      541 ggttaaccca acagaaggct cgagaaggta tatgctgttg acagtgagcg aaggacaagt
      601 atggtcatta aatagtgaag ccacagatgt atttaatgac catacttgtc ctgtgcctac
      661 tgcctcggat cccaaggggc tactttagga gcaattatct tgtttactaa aactgaatac
      721 cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa attaaatcac
      781 ttctagatgg acaactttga ctttgagaaa agagaggtgg aaatgaggaa a
//
