LOCUS       sequence-101718-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 
            for a translocation-based red fluorescent caspase-8 biosensor, used 
            together with plasmid 2 (BNLS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: RANLS-IETD-BNES
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(104..127)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(302..321)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     571..589
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(735..755)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(759..779)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(783..803)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atatcaagac ctgctaattt caaggctaac ttgtaccgct cgtccatgcc cagacggtgg
       61 cggccctcgg agcgcccgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      121 tccagcttgg tgtccacaca gtaatcgccg ggcagttgca cgggcttctt ggccatgtag
      181 atggtctcga actccaccag gtagtggccg ccgtccttca gcttcagggc ctgatagcta
      241 tggcccttca gcacgccgtc ttcggggtac agcatctcgg tggaggcctc ccagcccatg
      301 gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcgcat cttcaccttg
      361 cagatcagcg tgccgtcctg cagggaggag tcctgggtaa cggtcaccag accgccgtct
      421 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga agggcagctt cttgtaatcg
      481 gggatgtcgg cggggtgcct cacgtacgcc tcggagccgt acatgaactg gggggacagg
      541 atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc ggtctgggtg
      601 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcatggag
      661 ccctccatgc gcaccttgaa gcgcatgaac tctttgatgg tggatccatc cgtctcaatg
      721 ccggaggcgg tacctacctt tctcttcttt tttggatcta cctttctctt cttttttgga
      781 tctacctttc tcttcttttt tggatcgctt cccgccgttc ccttgtacag ctcgtccatg
      841 cccaggtggt ggcggccctc g
//
