LOCUS       sequence-101719-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 
            for a translocation-based red fluorescent caspase-9 biosensor, used 
            together with plasmid 2 (BNLS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: RANLS-LEHD-BNES
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(98..121)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(296..315)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     565..583
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(729..749)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(753..773)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(777..797)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agacctgcta atttcaaggc taacttgtac cgctcgtcca tgcccagacg gtggcggccc
       61 tcggagcgcc cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      121 ttggtgtcca cacagtaatc gccgggcagt tgcacgggct tcttggccat gtagatggtc
      181 tcgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggcctgata gctatggccc
      241 ttcagcacgc cgtcttcggg gtacagcatc tcggtggagg cctcccagcc catggtcttc
      301 ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac cttgcagatc
      361 agcgtgccgt cctgcaggga ggagtcctgg gtaacggtca ccagaccgcc gtcttcgaag
      421 ttcatcacgc gctcccactt gaagccctcg gggaagggca gcttcttgta atcggggatg
      481 tcggcggggt gcctcacgta cgcctcggag ccgtacatga actgggggga caggatgtcc
      541 caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg ggtgccctcg
      601 taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat ggagccctcc
      661 atgcgcacct tgaagcgcat gaactctttg atggtggatc catcatgctc taagccggag
      721 gcggtaccta cctttctctt cttttttgga tctacctttc tcttcttttt tggatctacc
      781 tttctcttct tttttggatc gcttcccgcc gttcccttgt acagctcgtc catgcc
//
