LOCUS       sequence-101720-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For imaging of ERK kinase activity using a single polypeptide RA-WW 
            domain-ERK substrate-B protein and free GA-NES..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: Ratiometric FPX sensor for ERK kinase activity
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(145..168)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(343..362)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     612..630
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattctta cttgtaccgc tcgtccatgc ccagacggtg gcggccctcg gagcgcccgt
      121 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gtgtccacac
      181 agtaatcgcc gggcagttgc acgggcttct tggccatgta gatggtctcg aactccacca
      241 ggtagtggcc gccgtccttc agcttcaggg cctgatagct atggcccttc agcacgccgt
      301 cttcggggta cagcatctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg
      361 ggccgtcggg ggggaagttg gtgccgcgca tcttcacctt gcagatcagc gtgccgtcct
      421 gcagggagga gtcctgggta acggtcacca gaccgccgtc ttcgaagttc atcacgcgct
      481 cccacttgaa gccctcgggg aagggcagct tcttgtaatc ggggatgtcg gcggggtgcc
      541 tcacgtacgc ctcggagccg tacatgaact ggggggacag gatgtcccag gcgaagggca
      601 gggggccgcc cttggtcacc ttcagcttgg cggtctgggt gccctcgta
//
