LOCUS       sequence-101721-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HTLV-1 based transfer vector encoding mcherry reporter gene 
            interrupted by intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, 
            Nikolaitchik O, Hu WS, Mazurov D
  TITLE     Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 
            Replication-Dependent Vectors via Optimization of Reporter Gene 
            Reconstitution and Modification with Intronic Short Hairpin RNA.
  JOURNAL   J Virol. 2015 Oct 15;89(20):10591-601. doi: 10.1128/JVI.01940-15. 
            Epub 2015 Aug 12.
  PUBMED    26269177
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01940-15"; journalName: "J Virol"; date: "2015-10-15- 
            15"; volume: "89"; issue: "20"; pages: "10591-601"
COMMENT     Sequence Label: pCRU5-inmCherry
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(316..335)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           682..802
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 gcggtcggcc cgcgtctagt cgacggatat ccacgcgtta cttgtacagc tcgtccatgc
       61 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      121 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      181 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      241 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      301 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      361 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
      421 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
      481 ggtgagtcca ggagatgttt cagcactgtt gcctttagtc tcgaggcaac ttagacaact
      541 gagtattgat ctgagcacag cagggtgtga gctgtttgaa gatactgggg ttgggggtga
      601 agaaactgca gaggactaac tgggctgaga cccagtggca atgttttagg gcctaaggaa
      661 tgcctctgaa aatctagatg tttgaatgag gcttcagtac tttacagaat cgttgcctgc
      721 acatcttgga aacacttgct gggattactt cttcaggtta acccaacaga aggctcgaga
      781 aggtatatgc tgttgacagt gagcgaagga caagtatggt cattaaa
//
