LOCUS       sequence-101722-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HIV-1 transfer vector that encodes mcherry reporter gene interrupted
            by intron .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, 
            Nikolaitchik O, Hu WS, Mazurov D
  TITLE     Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 
            Replication-Dependent Vectors via Optimization of Reporter Gene 
            Reconstitution and Modification with Intronic Short Hairpin RNA.
  JOURNAL   J Virol. 2015 Oct 15;89(20):10591-601. doi: 10.1128/JVI.01940-15. 
            Epub 2015 Aug 12.
  PUBMED    26269177
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JVI.01940-15"; journalName: "J Virol"; date: "2015-10-15- 
            15"; volume: "89"; issue: "20"; pages: "10591-601"
COMMENT     Sequence Label: pUCHR-inmCherry
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(312..331)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           678..798
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 agcggtcggc cgctttactt gtacatccac gcgttacttg tacagctcgt ccatgccgcc
       61 ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg
      121 ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt
      181 ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct
      241 ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc
      301 ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag
      361 cttcaccttg tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac
      421 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacaggtg
      481 agtccaggag atgtttcagc actgttgcct ttagtctcga ggcaacttag acaactgagt
      541 attgatctga gcacagcagg gtgtgagctg tttgaagata ctggggttgg gggtgaagaa
      601 actgcagagg actaactggg ctgagaccca gtggcaatgt tttagggcct aaggaatgcc
      661 tctgaaaatc tagatgtttg aatgaggctt cagtacttta cagaatcgtt gcctgcacat
      721 cttggaaaca cttgctggga ttacttcttc aggttaaccc aacagaaggc tcgagaaggt
      781 atatgctgtt gacagtgagc gaaggacaag tatggtcatt aaatagtgaa
//
