LOCUS       sequence-101724-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ddGFP A-NES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GA-NES
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(98..121)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(296..315)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     565..583
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      725..734
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agacctgcta atttcaaggc taaggtgctg ccggtgccat gccccaggtg gtggcggccc
       61 tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      121 ttggtgtcca cgaagtagta gccgggcagt tgcacgggct tcttggccat gtagatggtc
      181 ttggactcca ccaggttgag gccgccgtcc ttcagcttca ggcgccccag gagctcgccc
      241 ttcagcacgc cttcttcggg gtacaggcgc tcggtggcat aatcccagcc caaggtcttc
      301 ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac cttgtagatc
      361 agcgtgccgt cctgcaggga ggagtcctgt gtcgcggtca ccagaccgcc gtcctcgaag
      421 tgcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcatgta atcggggacg
      481 tcggcggggt gcttcacgta catcttggag ccgtacatga tcagggggga caggatgtcc
      541 caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg ggtgccctcg
      601 taagggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat ggagccctcc
      661 aagcgcacct tgaagcgcat gaactctttg atgacctcct cgctcttgct caccattctc
      721 gaggccatgg tggcggcacg cta
//
