LOCUS       sequence-101726-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  green ddFP based caspase-3 biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang 
            J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
  TITLE     Ratiometric biosensors based on dimerization-dependent fluorescent 
            protein exchange.
  JOURNAL   Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
  PUBMED    25622108
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261."
COMMENT     Sequence Label: GA-DEVD-GB-NES
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(112..135)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(310..329)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     579..597
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agctcccgat atcaagacct gctaatttca aggctaactt gtaccgctcg tccatgccca
       61 gacggtggcg gccctcggag cgcccgtact gttccacgat ggtgtagtcc tcgttgtggg
      121 aggtgatgtc cagcttggtg tccacacagt aatcgccggg cagttgcacg ggcttcttgg
      181 ccatgtagat ggtctcgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct
      241 gatagctatg gcccttcagc acgccgtcct tggggtacag catctcggtg gaggcctccc
      301 agcccatggt cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcgaatct
      361 tcaccttgca gatcagcgtg ccgtcctgca gggaggagtc ctgggtaacg gtcaccagac
      421 cgccgtcttc gaagttcatc acgcgctccc acttgaagcc ctcggggaag ggcagcttct
      481 tgtaatcggg gatgtcggcg gggtgcctca cgtacgcttt ggagccgtac atgatctggg
      541 gggacaggat gtcccaggca aagggcaggg ggccgccctt ggtcaccttc agcttggcgg
      601 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
      661 tcatggagcc ctccatgcgc accttgaagc gcatgaactc tttgatggtg gctccatcca
      721 cctcatcgcc ggaggcggta ccggagctac cgctgcctgt gctacctgta ccatgcccca
      781 ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgta
//
