LOCUS       sequence-101732-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Wu J, Prole DL, Shen Y, Lin Z, Gnanasekaran A, Liu Y, Chen L, Zhou 
            H, Chen SR, Usachev YM, Taylor CW, Campbell RE
  TITLE     Red fluorescent genetically encoded Ca2+ indicators for use in 
            mitochondria and endoplasmic reticulum.
  JOURNAL   Biochem J. 2014 Nov 15;464(1):13-22. doi: 10.1042/BJ20140931.
  PUBMED    25164254
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2014-11-15- 15"; volume: "464"; issue: "1"; 
            pages: "13-22"
COMMENT     Sequence Label: CMV-ER-LAR-GECO1
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagaagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagcttgg taccgagctc ggatccaccg ccatgctgct gcccgtcccc ctgctgctgg
      121 gcctgctggg cgccgccgcc gacgtcgact cttcacgtcg taagtggaat aaggcaggtc
      181 acgcatggag agctataggt cggctgagct cacccgtggt ttccgagcgg atgtaccccg
      241 aggacggagc cctgaagagc gagatcaaga aggggctgag gctgaaggac ggcggccact
      301 acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      361 acgtcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc atcgtggaac
      421 agtgcgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggag
      481 gtacaggcgg gagtctggtg agcaagggcg aggaggataa catggccatc atcaaggagt
      541 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
      601 gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag gtgaccaagg
      661 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
      721 cctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc c
//
