LOCUS       sequence-101734-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses LAR-GECO1.2 in the mitochondria in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Wu J, Prole DL, Shen Y, Lin Z, Gnanasekaran A, Liu Y, Chen L, Zhou 
            H, Chen SR, Usachev YM, Taylor CW, Campbell RE
  TITLE     Red fluorescent genetically encoded Ca2+ indicators for use in 
            mitochondria and endoplasmic reticulum.
  JOURNAL   Biochem J. 2014 Nov 15;464(1):13-22. doi: 10.1042/BJ20140931.
  PUBMED    25164254
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2014-11-15- 15"; volume: "464"; issue: "1"; 
            pages: "13-22"
COMMENT     Sequence Label: CMV-mito-LAR-GECO1.2
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..175
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             197..271
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cgggccctct agactcgagc atgtctgttc tgactcctct
      121 gctgctccgg ggtctcacag gttccgcaag aagactcccc gtgcctaggg ccaaaattca
      181 ttcactgggg gaccccatga gcgtgctcac cccactcctg ctgcgggggc tgaccggcag
      241 cgctaggcgg ctgccagtcc ccagggccaa gatccacagt ctcggcgatc ccaaggatcc
      301 aaccatggtc gactcttcac gtcgtaagtg gattaagcgt ggtcacgcag tcagagctat
      361 aggtcggctg agctcacccg tggtttccga gcggatgtac cccgaggacg gcgctctgaa
      421 gagcgagatc aagaaggggc tgaggctgaa ggacggcggc cactacgccg ccgaggtcaa
      481 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacatag tcgacatcaa
      541 gttggacatc gtgtcccaca acgaggacta caccatcgtg gaacagtgcg aacgcgccgt
      601 gggccgccac tccaccggcg gcatggacga gctgtacaag ggaggtacag gcgggagtct
      661 ggtgagcaag ggcgaggagg ataacagggc catcgtcaag gagttcatgc gcttcaagtt
      721 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      781 cccctacgag gcctttcaga ccgctaagct gaaggtgacc aa
//
