LOCUS       sequence-101740-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1 kb zebrafish insulin promoter driving eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   diIorio PJ, Moss JB, Sbrogna JL, Karlstrom RO, Moss LG
  TITLE     Sonic hedgehog is required early in pancreatic islet development.
  JOURNAL   Dev Biol. 2002 Apr 1;244(1):75-84.
  PUBMED    11900460
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2002-04-1"; volume: "244(1)"; pages: "75-84"
COMMENT     Sequence Label: pKSII/INSeGFP
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..43
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     72..88
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     73..89
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac
       61 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgctaatc agcccacagt
      121 ctagtttagt tgttggtccc ttatgagaaa aagtttgggc acctctgctc tagtgcttgt
      181 aaacattttt gagtatctat agttgaacat gaaagcattt tttttttttt taagaaaatg
      241 aagaaagcta aaaacctgta tccactgact tccataaaaa agcaaatact agggatgtct
      301 atggtgtctt cttttgtgtt caaccaataa acagaaagta actgctgaca gaatattaca
      361 ttttgtgtga atccccttaa attgtgttaa aaactgtaga gaaatcatct aataagaaga
      421 aattatcaag aaataaacag aaaatgatct aatagtatac tataaaatct ttcaaaatcc
      481 aggtcactac cctatgacta aaaccacaca tattaggttt taaaaaaaag tcacaatgaa
      541 gacgtcacaa acgttcctga agactaatga ctcgtctaat cccatcttac cttctgctgt
      601 ttcactcaaa gaaaagtcac cagctgtctc tgtgatctgc tcattctgtg taaacttgtg
      661 ttctcaacaa ttaacaatta accaaaagcc tctggagctg cgtgagcatc acgatgtgtt
      721 tgtcttctgt gagcgtggca gtgtccacga agtcaatgtg aattggttgg ccaaacacag
      781 ctaatgtggc atgagtgatt ggacagccac ttaaaagtgt taaagtgtgt ttacgcacag
      841 gattggtttt ccgctgaatc aggcaaaacc agcagaaccg atctaaactc gagtataaaa
      901 gtgagtagga gatgctattc gtcccaaaac atctccacca ccatatccac cattcctcgc
      961 ctctgcttcg agaacaggtg agtgtcgagc gggatggtaa atctacagag aatgcggagt
     1021 gtagcttgtg tacatgtttt tgattaacag agattgtatg tgtgtgtttg tgtcagtgtg
     1081 accatg
//
