LOCUS       sequence-101741-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  1 kb zebrafish insulin promoter driving eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   diIorio PJ, Moss JB, Sbrogna JL, Karlstrom RO, Moss LG
  TITLE     Sonic hedgehog is required early in pancreatic islet development.
  JOURNAL   Dev Biol. 2002 Apr 1;244(1):75-84.
  PUBMED    11900460
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2002-04-1"; volume: "244(1)"; pages: "75-84"
COMMENT     Sequence Label: pKSII/INSeGFP
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(194..213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(195..213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(223..240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(223..239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(232..254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      381..836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(468..487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     678..699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        862..966
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagatcag atctggttct atagtgctcg agatatctta agccgaattc
      181 gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
      241 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      301 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      361 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      601 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      661 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      721 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      781 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
      841 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      901 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      961 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1021 cttcctgttt ttgctcaccc agaaacgctg gt
//
