LOCUS       sequence-101744-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses REX-GECO0.9 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A,
            Zhou H, Ballanyi K, Wicks G, Drobizhev M, Rebane A, Ruthazer ES, 
            Campbell RE
  TITLE     A long Stokes shift red fluorescent Ca(2+) indicator protein for 
            two-photon and ratiometric imaging.
  JOURNAL   Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262.
  PUBMED    25358432
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-31"; volume: "5:5262. doi"; pages: " 
            10.1038/ncomms6262"
COMMENT     Sequence Label: CMV-REX-GECO0.9
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..160
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccaccat ggtcgactca tcacgtcgta agtggaataa
      121 ggcaggtcac gcagtcagag ctataggtcg gctgagctca cgttgggttt ccgagtggat
      181 gtaccccgag gacggcgccc tgaagagcgt gatcaaggag gggctgaggc tgaaggacgg
      241 cggccactac gccgccgagg tcaggaccac ctacaaggcc aaaaagcccg tgcagctgcc
      301 cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc cacaacgagg actacaccat
      361 cgtggaacag tgcgaacgcg ccgtgggccg ccaccccacc ggcggcatgg tcgagctgta
      421 caagggaggt acaggcggga gtctggtgag caagggcgag gaggataaca tggccatcat
      481 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      541 gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt
      601 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccttcagt tcatgtacgg
      661 ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagc tgtccttccc
      721 cgagggcttc aggtgggagc gcgtgatgat cttcgaggac ggcggcatta ttcacgttga
      781 ccaggactcc tccctgcagg acggcgtatt catctacaa
//
