LOCUS       sequence-101746-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a guide RNA (gRNA) to target human IRF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: gRNA-hIRF-1 #12/pSIR
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        65..140
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(152..176)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcgggg gcgctgggct
       61 gtccgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      121 aagtggcacc gagtcggtgc tttttttcta gacccagctt tcttgtacaa agttggcatt
      181 aaagggcgaa ttccagcaca ctggcggccg ttactagtgg atccgagctc ggtaccaagc
      241 ttgtcgacgg atccgaattc gtcgagggaa gaaataataa agacattaaa atcacttttg
      301 taaacttttt aaataaggga aattgattaa aacaacgcga agttgtaaaa tgccatatac
      361 tgcatacatt ttgtgggaaa aaatgtgcat ttacctgctc accaaattat cttattagct
      421 agcagtagag ctctttgtgt gatgggaaga tgggataaca gctgttgtta ctgtactggt
      481 tttttttttt aatatattta aaaaaaagag tgtctcctaa agtgtaagcg ctatcatgct
      541 ggactaatgt tttaatttaa tttaattgtt gtcttggtag agatggggtc tcgctatttt
      601 gctcacgcag atctcaaact cctgacgccc aggtacactc ccatctcggc ctcctaaaat
      661 ggtgggatca cagacgtcag ccaccgagcc cggtcacttt tttgtattcc ccacagtatt
      721 gatgtatatc ttctgcgttc aaaagcaatt ttttaaagcc tcataacgtg gtaacagaat
      781 actttgcaca ttacaaaatt cagaacacgg aaacaagaag ctcgcttttt tttcccccct
      841 atttcggttt ggccctttag atttcccctc ccccaccggg gcgggacttc ccgccgactt
      901 ctttcaggtt ctcagttcgg tccgccaact gtcgtataaa ggcgctgcct caggccagag
      961 gcctcacaaa gcgcgtcgag acagcaagcg aaccggaatt gccagctggg gcgccctctg
     1021 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     1081 ggcgcaggg
//
