LOCUS       sequence-101750-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of IFT88 fused with N terminal TagRFP.T tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
  TITLE     Single molecule imaging reveals a major role for diffusion in the 
            exploration of ciliary space by signaling receptors.
  JOURNAL   Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.
  PUBMED    23930224
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2013-08-6"; volume: "2:e00654. doi"; pages: " 
            10.7554/eLife.00654. Print 2013"
COMMENT     Sequence Label: pEF5-FRT-TagRFP-T-IFT88
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..827
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    864..888
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttcaggtgt cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag
       61 ctggctaggt aagcttggta ccgagctcgg atccgatggt gtctaagggc gaagagctga
      121 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
      181 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      241 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
      301 gcagcagaac cttcatcaac cacacccagg gcatccccga tttctttaag cagtccttcc
      361 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
      481 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg
      541 agatgctgta ccccgctgac ggcggcctgg aaggcagaac cgacatggcc ctgaagctcg
      601 tgggcggggg ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta
      661 agaacctcaa gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg
      721 ccgacaaaga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
      781 ctagcaaact ggggcacaaa cttaatggca tggacgagct gtacaaggga ctagtccagt
      841 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agcaggcacc atggaaaatg
      901 ttcatctggc accagaaaca gatgaggacg acctttactc tggtttcaac gactacaacc
      961 cagcctatga tactgaggag ttggagaatg acacgggctt tcagcaagca gtgagaacca
     1021 gtcatggcag aagacctccc gtaactgcta aaataccaag cacagcagtc agcaggccca
     1081 cagctactgg atatgggtcc a
//
