LOCUS       sequence-101751-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of IFT88 fused with N terminal TagRFP.T tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
  TITLE     Single molecule imaging reveals a major role for diffusion in the 
            exploration of ciliary space by signaling receptors.
  JOURNAL   Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.
  PUBMED    23930224
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2013-08-6"; volume: "2:e00654. doi"; pages: " 
            10.7554/eLife.00654. Print 2013"
COMMENT     Sequence Label: pEF5-FRT-TagRFP-T-IFT88
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact taatggcatg
       61 gacgagctgt acaagggact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
      121 tacaaaaaag caggcaccat ggaaaatgtt catctggcac cagaaacaga tgaggacgac
      181 ctttactctg gtttcaacga ctacaaccca gcctatgata ctgaggagtt ggagaatgac
      241 acgggctttc agcaagcagt gagaaccagt catggcagaa gacctcccgt aactgctaaa
      301 ataccaagca cagcagtcag caggcccaca gctactggat atgggtccaa gacatccctg
      361 acatcatcaa tgggaagacc gatgacaggg acaattcagg atggagttgc tcgaccgatg
      421 acagcagtga gagcagctgg cttttccaag gcagctttgc gaggctctgc atttgacccc
      481 cttggtcagt ccaggggccc tgctcctcct ttggaagcca aaaatgaaga cagtccagaa
      541 gaaaaaatta gacagttgga gaagaaagta aatgagttgg tggaagaaag ctgtattgcc
      601 aacagttgtg gagacttaaa actggcctta gagaaggcaa aagatgcagg aagaaaagag
      661 agagtcctgg tgagacaacg agagcaagtc acaagtccag aaaatatcaa cttggactta
      721 acctactccg ttcttttcaa cttggccagt cagtattctg ctaatgaaat gtatgcagaa
      781 gcactgaaca cataccaagt tatagtgaaa aacaagatgt ttagcaatgc aggaagactg
      841 aaagtgaata tgggaaatat tcatttaaag cagagaaatt attccaaagc cattaagttc
      901 taccgaatgg ccttagatca gatcccaagt gtccataaag aaatgaggat caaaataatg
      961 cagaacattg gaatcacatt tataaaaact ggtcagtact cagatg
//
