LOCUS       sequence-101752-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of IFT88 fused with N terminal TagRFP.T tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
  TITLE     Single molecule imaging reveals a major role for diffusion in the 
            exploration of ciliary space by signaling receptors.
  JOURNAL   Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.
  PUBMED    23930224
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2013-08-6"; volume: "2:e00654. doi"; pages: " 
            10.7554/eLife.00654. Print 2013"
COMMENT     Sequence Label: pEF5-FRT-TagRFP-T-IFT88
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..62)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    115..139
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
       61 ccttcgaacc gcgggccctc tagactcgag cggccgccac tgtgctggat atcaaccact
      121 ttgtacaaga aagctgggtt ctattctggg agcaagtcat ctcctaactc ttcatcagca
      181 aaatcatcct cgtcaattct tttcttggct gcagtttttg gtctttctat ctgagggccc
      241 agtggatcca cgtaggaggc atctatttct ttgttaccgc tactttcata aggttcatct
      301 gtcccaggca aagctctgag cttggcactc agtcgttcac tcttactact ggcactattg
      361 ttctggccac tgtcactgcc agcactcccc tctcgtttgc tgcgagaacc cccgctgctg
      421 tctctgcccg attttatgcg ctgttccctc atttctttca ttttttccaa cctcttgagt
      481 tttgtggcat attcttgaac ttcttttaat ccaatctctg tgcagagacg aaccaagaaa
      541 cgcaaacatt caacattctc tggaaatttc ctgtgaatct ctttataagt atctaatgcc
      601 ttttggtagt taccacttcg tctaaagcag ctagctacca tcagctgcca cttcacttgt
      661 gtgggttgta taagggaagc tctttcaaag tactgaatgg ctttctcgca gaactgtgta
      721 tcaatgtaat aagctccaag ccattcaatg acttcaatgt tagaagggaa atacctgtat
      781 gactcatagt aatattggaa tgcctgggac ttgtctccct cactatcgta taactctccc
      841 agtttagaca gagcttggga atcagttgga acaacgctga tcaactgcat cagccattca
      901 atagcttgat tgggatcttc cattaactca tatatgtttg ctatctggca aagaacttga
      961 gcgctgttcc gaaggattgc gtgcagtttc aggaaggagt ccaaggcttc atccagacgg
     1021 tttagcttct tataggtgag gccaatgtta tacagtgctt cagtacagga agagtcgttt
     1081 ctcagggcct ct
//
