LOCUS       sequence-101753-        9086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of IFT88 fused with N terminal TagRFP.T tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Ye F, Breslow DK, Koslover EF, Spakowitz AJ, Nelson WJ, Nachury MV
  TITLE     Single molecule imaging reveals a major role for diffusion in the 
            exploration of ciliary space by signaling receptors.
  JOURNAL   Elife. 2013 Aug 6;2:e00654. doi: 10.7554/eLife.00654. Print 2013.
  PUBMED    23930224
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2013-08-6"; volume: "2:e00654. doi"; pages: " 
            10.7554/eLife.00654. Print 2013"
COMMENT     Sequence Label: pEF5-FRT-TagRFP-T-IFT88
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        353..1531
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          584..1522
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1479..1499
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1548..1567
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1548..1566
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1612..2343
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     protein_bind    2380..2404
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4883..4907)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4960..5001
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5039..5056)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5045..5269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5402..5421)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5552..5599
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5607..6629
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5607..6629
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6759..6880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6796..6815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6850..6869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6929..6945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6929..6945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6942..6964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6953..6969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6977..6983,6984..7001,7002..7007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7022..7043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7160..7177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7331..7919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7411..7430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8090..8881,8882..8950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8713..8732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8951..9055)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgcctcgt gaggctccgg
      361 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      421 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      481 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      541 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      601 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      661 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      721 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      781 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      841 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
      901 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
      961 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     1021 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     1081 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1141 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1201 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1261 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1321 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1381 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1441 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1501 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac
     1561 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc gatggtgtct
     1621 aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     1681 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     1741 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     1801 accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgatttc
     1861 tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     1921 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     1981 aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc
     2041 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaaccgac
     2101 atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga
     2161 tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg
     2221 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc
     2281 agatactgcg acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac
     2341 aagggactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2401 ggcaccatgg aaaatgttca tctggcacca gaaacagatg aggacgacct ttactctggt
     2461 ttcaacgact acaacccagc ctatgatact gaggagttgg agaatgacac gggctttcag
     2521 caagcagtga gaaccagtca tggcagaaga cctcccgtaa ctgctaaaat accaagcaca
     2581 gcagtcagca ggcccacagc tactggatat gggtccaaga catccctgac atcatcaatg
     2641 ggaagaccga tgacagggac aattcaggat ggagttgctc gaccgatgac agcagtgaga
     2701 gcagctggct tttccaaggc agctttgcga ggctctgcat ttgaccccct tggtcagtcc
     2761 aggggccctg ctcctccttt ggaagccaaa aatgaagaca gtccagaaga aaaaattaga
     2821 cagttggaga agaaagtaaa tgagttggtg gaagaaagct gtattgccaa cagttgtgga
     2881 gacttaaaac tggccttaga gaaggcaaaa gatgcaggaa gaaaagagag agtcctggtg
     2941 agacaacgag agcaagtcac aagtccagaa aatatcaact tggacttaac ctactccgtt
     3001 cttttcaact tggccagtca gtattctgct aatgaaatgt atgcagaagc actgaacaca
     3061 taccaagtta tagtgaaaaa caagatgttt agcaatgcag gaagactgaa agtgaatatg
     3121 ggaaatattc atttaaagca gagaaattat tccaaagcca ttaagttcta ccgaatggcc
     3181 ttagatcaga tcccaagtgt ccataaagaa atgaggatca aaataatgca gaacattgga
     3241 atcacattta taaaaactgg tcagtactca gatgccatca actcatttga gcacatcatg
     3301 agcatggccc cgagcctgaa ggcaggcttc aacctcatcc ttagttgttt tgccattgga
     3361 gatcgggaga aaatgaagaa ggcattccaa aaattaattg ctgttccgtt agaaattgat
     3421 gaagatgata aatatatctc cccaagtgat gatccccata ctaacttgct gattgaagct
     3481 ataaaaaatg accaccttag gcaaatggaa cgtgaaagga aagccatggc agaaaaatac
     3541 atcatgacag ctgcaaaact cattgctcct gtgatcgaag cgtcttttgc tgtgggttat
     3601 aactggtgtg tggaagtagt gaaagcctct cagtatgtgg agctggccaa cgacctggag
     3661 attaacaaag caattactta cttgagacaa aaggacttta accaagctgt agacacattg
     3721 aaaatgtttg agaagaagga cagtagagtg aagagtgcag ctgcgaccaa cctctcgttc
     3781 ctgtattatc tggaaaacga atttgcccaa gccagcagct atgcagattt agctgtgaac
     3841 tcggatagat ataacccgtc agctcttact aataaaggga acacagtttt tgcaaatggt
     3901 gattatgaga aggcagctga gttctataaa gaggccctga gaaacgactc ttcctgtact
     3961 gaagcactgt ataacattgg cctcacctat aagaagctaa accgtctgga tgaagccttg
     4021 gactccttcc tgaaactgca cgcaatcctt cggaacagcg ctcaagttct ttgccagata
     4081 gcaaacatat atgagttaat ggaagatccc aatcaagcta ttgaatggct gatgcagttg
     4141 atcagcgttg ttccaactga ttcccaagct ctgtctaaac tgggagagtt atacgatagt
     4201 gagggagaca agtcccaggc attccaatat tactatgagt catacaggta tttcccttct
     4261 aacattgaag tcattgaatg gcttggagct tattacattg atacacagtt ctgcgagaaa
     4321 gccattcagt actttgaaag agcttccctt atacaaccca cacaagtgaa gtggcagctg
     4381 atggtagcta gctgctttag acgaagtggt aactaccaaa aggcattaga tacttataaa
     4441 gagattcaca ggaaatttcc agagaatgtt gaatgtttgc gtttcttggt tcgtctctgc
     4501 acagagattg gattaaaaga agttcaagaa tatgccacaa aactcaagag gttggaaaaa
     4561 atgaaagaaa tgagggaaca gcgcataaaa tcgggcagag acagcagcgg gggttctcgc
     4621 agcaaacgag aggggagtgc tggcagtgac agtggccaga acaatagtgc cagtagtaag
     4681 agtgaacgac tgagtgccaa gctcagagct ttgcctggga cagatgaacc ttatgaaagt
     4741 agcggtaaca aagaaataga tgcctcctac gtggatccac tgggccctca gatagaaaga
     4801 ccaaaaactg cagccaagaa aagaattgac gaggatgatt ttgctgatga agagttagga
     4861 gatgacttgc tcccagaata gaacccagct ttcttgtaca aagtggttga tatccagcac
     4921 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     4981 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttaaacccg ctgatcagcc
     5041 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5101 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5161 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5221 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5281 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5341 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5401 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5461 ctaaatcggg ggtcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5521 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc
     5581 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga
     5641 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     5701 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     5761 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     5821 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     5881 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     5941 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     6001 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     6061 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     6121 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     6181 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     6241 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     6301 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     6361 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     6421 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     6481 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     6541 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     6601 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg
     6661 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6721 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6781 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6841 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6901 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6961 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7021 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7081 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7741 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9061 ttccccgaaa agtgccacct gacgtc
//
