LOCUS       sequence-101754-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct N-terminal GFP fusions under control of a Zn2+ 
            inducible promoter, integrates via double crossover at bgaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pJWV25
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..733)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtcctgtgga tctatcgatg catgcatcgg tacctgcgaa ttctagatgg cttttttggt
       61 tttgctgata cggcggtcaa tgttagtcat atggacactt aaggcaaatt gttcagaact
      121 gaataaagct gacgttttgc ttctatcctt tctttgagtt ttagtggata atgataatga
      181 acaaggtgtt cataaatcta ttataacaaa ggaatgagaa atatgaaggc aaaatatgct
      241 gtttgggtgg cttttttctt aaatttgact tatgccattg ttgagtttat tgcaggtgga
      301 gtatttggtt ctagcgctgt tcttgctgac tctgtgcatg acttgggaga tgcgattgca
      361 attggaatat cagcttttct agaaacaatc tccaatcgtg aagaagacaa tcagtacacc
      421 ttgggctata agcggtttag cctgctagga gccttagtaa cagctgtgat tctcgtaacg
      481 ggctctgttc tagtcatttt ggaaaatgtc acgaagattt tgcatccgca accagtcaat
      541 gatgagggga ttctctggtt aggaattatt gcgattacta tcaatctgtt aacgagtctg
      601 gtggttggta agggaaagac aaagaatgag tctattctga gtctgcggat cttaaggagg
      661 caaatatgaa acatcttagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
      721 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      781 atgctacata cggaaagctt acccttaa
//
