LOCUS       sequence-101755-         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To construct N-terminal GFP fusions under control of a Zn2+ 
            inducible promoter, integrates via double crossover at bgaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW
  TITLE     Cellular localization of choline-utilization proteins in 
            Streptococcus pneumoniae using novel fluorescent reporter systems.
  JOURNAL   Mol Microbiol. 2009 Oct;74(2):395-408. doi: 
            10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8.
  PUBMED    19737355
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2958.2009.06872.x"; journalName: "Mol Microbiol"; 
            date: "2009-10"; volume: "74"; issue: "2"; pages: "395-408"
COMMENT     Sequence Label: pJWV25
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaagttcttc tcctttgcta agatgtttca tatttgcctc cttaagatcc gcagactcag
       61 aatagactca ttctttgtct ttcccttacc aaccaccaga ctcgttaaca gattgatagt
      121 aatcgcaata attcctaacc agagaatccc ctcatcattg actggttgcg gatgcaaaat
      181 cttcgtgaca ttttccaaaa tgactagaac agagcccgtt acgagaatca cagctgttac
      241 taaggctcct agcaggctaa accgcttata gcccaaggtg tactgattgt cttcttcacg
      301 attggagatt gtttctagaa aagctgatat tccaattgca atcgcatctc ccaagtcatg
      361 cacagagtca gcaagaacag cgctagaacc aaatactcca cctgcaataa actcaacaat
      421 ggcataagtc aaatttaaga aaaaagccac ccaaacagca tattttgcct tcatatttct
      481 cattcctttg ttataataga tttatgaaca ccttgttcat tatcattatc cactaaaact
      541 caaagaaagg atagaagcaa aacgtcagct ttattcagtt ctgaacaatt tgccttaagt
      601 gtccatatga ctaacattga ccgccgtatc agcaaaacca aaaaagccat ctagaattcg
      661 caggtaccga tgcatgcatc gatagatcca caggacgggt gtggtcgcca tgatcgcgta
      721 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
      781 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagccaagt
      841 aatcgtgaat gtcgctgtga aatatttaga tcaagaaaca aaggcaaccc aaatctcgca
      901 atttgagttg atattagaa
//
