LOCUS       sequence-101756-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRNA synthesis of tol1 transposase codon modified for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2-Tol1.zf1
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt
      121 cgaattcgag ctcggtacct cgcgaatgca tctagatacc caatgccatc atggaaaaaa
      181 aacgatcaaa accctcaggc gcgcaattca gaaagaagag aaaggaggag gaggagaaga
      241 gagacaagga gaagggcgct ctgctgagat actttggatc tagcaccact gctcaggatg
      301 agaccagcac ctctctgcct gccatctctt ctgccactgt gactgtgtct cctcctcagg
      361 atgagctgcc tagcacctct tctgccaccc atgtggtgcc tcagctgctg cctgagcagt
      421 cttttgactc tgaggctgag gatgtggtgc ctagcaccag cacccagctg gagacctctg
      481 agatgcctgg agatgagacc cctctgaccc caactgctga ggaccagcct ctgccaactg
      541 accctgccaa gtggccttct cctctgactg acagaatcag aatggagctg gtgagaagag
      601 gaccttctag catccctcct gactttgtgt tcccaagaaa tgactctgat ggacgctctt
      661 gccaccacca ctacttcaga aagaccctgg tgtctggaga gaagattgcc agaacctggc
      721 tgatgtacag caaggtgaag aactctctgt tctgcttctg ctgcaagctg ttcagcaaca
      781 agaacatcaa cctgaccacc tctggaactg ccaactggaa gcatgctagc acctacctga
      841 ctgctcatga gaagtctcct gagcacctga actgcatgaa ggcttggaag gagctgtctg
      901 gaagaatccg ctctggaaag accattgaca agcaggagat ggctctgctg gaggaggaga
      961 gagtgagatg gagagctgtg ctgaccagac tgattgccat tgtgcagtct ctggctgtga
     1021 gaaacctggc tctgagagga cacactgaga ccctgttca
//
