LOCUS       sequence-101760-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AMPK biosensor targeted to the endoplasmic reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, 
            Chen M, Tokumitsu H, Zhang J, Inoue T
  TITLE     Compartmentalized AMPK signaling illuminated by genetically encoded 
            molecular sensors and actuators.
  JOURNAL   Cell Rep. 2015 Apr 28;11(4):657-70. doi: 
            10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16.
  PUBMED    25892241
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.03.057"; journalName: "Cell Rep"; date: 
            "2015-04-28- 28"; volume: "11"; issue: "4"; pages: "657-70"
COMMENT     Sequence Label: ER-ABKAR
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     451..472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(578..599)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(839..858)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcatttccaa atactactag gagaagacgg taacctttta ttgaatgaca tttccactaa
       61 tgggacctgg ttaaatgggc aaaaagtcga gaagaacagc aatcagttac tgtctcaagg
      121 tgatgaaata accgttggtg taggcgtgga atcagatatt ttatctctgg tcattttcat
      181 aaacgacaaa tttaagcagt gcctcgagca gaacaaagtt gatcgctctg caggtaagcc
      241 aggcagcggc gagggcagca ccaagatgcg tcgtgtcgcc accctggtgg acctcggcac
      301 cggcggcagc gagctcatgg gcggcgtgca gctcgccgac cactaccagc agaacacccc
      361 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
      421 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      481 cgggatcact ctcggcatgg acgagctgta caagggaggt accggtggat ctatggtgag
      541 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      601 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      661 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      721 caccctgggc tacggccttc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      781 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      841 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      901 catcgagctg aagggcatcg acttcaagga ggacggcaac atcct
//
