LOCUS       sequence-101762-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRNA synthesis of cerulean codon modified for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2-Cer.zf1
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    868..1002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(892..911)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     946..965
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgccaccat
      121 ggtgtcaaaa ggagaggaac tgtttactgg agtcgtgcct atcctggtgg agctggatgg
      181 agatgtgaat ggacacaagt tctctgtgtc tggagaggga gagggagatg ctacatatgg
      241 aaagctgaca ctgaagttca tctgtacaac aggaaagctg cctgtgcctt ggcctacact
      301 ggtgacaaca ctgacatggg gagtgcagtg ttttgctaga taccctgacc acatgaagca
      361 gcatgacttc ttcaagtctg ctatgcctga gggatatgtg caggagagaa caatcttctt
      421 caaggatgat ggaaactaca agacaagagc tgaggtgaag tttgagggag acacactggt
      481 gaacagaatt gagctgaagg gaattgactt caaggaggat ggaaacatcc tgggacacaa
      541 gctggagtac aatgctatct ctgacaatgt gtacatcaca gctgacaagc agaagaatgg
      601 aatcaaggct aacttcaaga tcagacacaa cattgaggat ggatctgtgc agctggctga
      661 ccactaccag cagaacacac ctattggaga tggacctgtg ctgctgcctg acaaccacta
      721 cctgagcaca cagtctgctc tgagcaagga ccctaatgag aagagagacc acatggtgct
      781 gctggagttt gtgacagctg ctggaatcac actgggaatg gatgagctgt acaagtaaac
      841 tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
      901 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      961 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1021 cattttatgt t
//
