LOCUS       sequence-101768-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, 
            Arolas JL, Gomis-Ruth FX
  TITLE     The pCri System: A Vector Collection for Recombinant Protein 
            Expression and Purification.
  JOURNAL   PLoS One. 2014 Nov 11;9(11):e112643. doi: 
            10.1371/journal.pone.0112643. eCollection 2014.
  PUBMED    25386923
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-11-11"; volume: "9(11):e112643. doi"; pages: " 
            10.1371/journal.pone.0112643. eCollection 2014"
COMMENT     Sequence Label: pCri-12b
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acgagctgta caagtaaggt accggatccg aattcgagct ccgtcgacaa gcttgcggcc
       61 gcactcgagc atcatcatca tcatcactga ctgaaaagaa cgggaagatt tatgaagaaa
      121 ggttttatgt tgtttacttt gttagcggcg ttttcaggct ttgctcaggc tgatgacgcg
      181 gcaattcaac aaacgttagc caaaatgggc atcaaaagca gcgatattca gcccgcgcct
      241 gtagctggca tgaagacagt tctgactaac agcggcgtgt tgtacatcac cgatgatggt
      301 aaacatatca ttcaggggcc aatgtatgac gttagtggca cggctccggt caatgtcacc
      361 aataagatgc tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg
      421 ccgcaggaaa aacacgtcat caccgtgttt actgatatta cctgtggtta ctgccacaaa
      481 ctgcatgagc aaatggcaga ctacaacgcg ctggggatca ccgtgcgtta tcttgctttc
      541 ccgcgccagg ggctggacag cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa
      601 gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc agccagttgc
      661 gacgtggata ttgccgacca ttacgcactt ggcgtccagc ttggcgttag cggtactccg
      721 gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc agccgccgaa agagatgaaa
      781 gaattcctcg acg
//
