LOCUS       sequence-101769-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRNA synthesis of TagRFPT codon modified for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2-TagRFPT.zf1
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(928..947)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     982..1001
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgccaccat
      121 ggtgagcaaa ggagaggaac tgattaaaga aaacatgcac atgaagctgt acatggaggg
      181 aacagtgaac aaccaccact tcaagtgtac atctgaggga gagggaaagc cttatgaggg
      241 aacacagaca atgagaatca aggtggtgga gggaggacct ctgccttttg cttttgacat
      301 cctggctacc agcttcatgt atggaagcag aacattcatc aaccacacac agggaatccc
      361 tgacttcttc aagcagagct tccctgaggg attcacatgg gagagagtga caacatatga
      421 ggatggagga gtgctgacag ctacacagga cacaagcctc caggatggat gtctgatcta
      481 caatgtgaag atcagaggag tgaacttccc tagcaatgga cctgtgatgc agaagaagac
      541 actgggatgg gaggctaaca cagagatgct gtaccctgct gatggaggac tggagggaag
      601 aacagacatg gctctgaagc tggtgggagg aggacacctg atctgtaact tcaagacaac
      661 atacagaagc aagaagcctg ctaagaacct gaagatgcct ggagtgtact atgtggacca
      721 cagactggag agaatcaagg aggctgacaa ggagacatat gtggagcagc atgaggtggc
      781 tgtggctaga tactgtgacc tgcctagcaa gctgggacac aagctgaatg gaatggatga
      841 gctgtacaag taaaaattca aggcctctcg agcctctaga actatagtga gtcgtattac
      901 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      961 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccatt
//
