LOCUS       sequence-101772-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRNA synthesis of TagRFPT codon modified for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2-TagRFPT.zf1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    133..154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(169..174,175..192,193..199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     212..234
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     231..247
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     231..247
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     266..286
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        268..286
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    399..533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(436..455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     490..509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
       61 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      121 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
      181 gctcccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      241 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctggg
      301 taccgggccc aatgcattgg cgccgcggcc gcgaattaaa aaacctccca cacctccccc
      361 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
      421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctacgtaa
      541 tacgactcac tatagttcta gaggctcgag aggccttgaa tttttacttg tacagctcat
      601 ccattccatt cagcttgtgt cccagcttgc taggcaggtc acagtatcta gccacagcca
      661 cctcatgctg ctccacatat gtctccttgt cagcctcctt gattctctcc agtctgtggt
      721 ccacatagta cactccaggc atcttcaggt tcttagcagg cttcttgctt ctgtatgttg
      781 tcttgaagtt acagatcagg tgtcctcctc ccaccagctt cagagccatg tctgttcttc
      841 cctccagtcc tccatcagca gggtacagca tctctgtgtt agcctcccat cccagtgtct
      901 tcttctgcat cacaggtcca ttgctaggga agttcactcc tctgatcttc acattgtaga
      961 tcagacatcc atcctggagg cttgtgtcct gtgtagctgt cagcactcct ccatcctcat
     1021 atgttgtcac tctctcccat gtgaatccct cagggaagct ctgct
//
