LOCUS       sequence-101781-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a nuclear-targeted actin filament reporter, consisting of 
            the first 230 amino acids of human utrophin, on a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Belin BJ, Cimini BA, Blackburn EH, Mullins RD
  TITLE     Visualization of actin filaments and monomers in somatic cell 
            nuclei.
  JOURNAL   Mol Biol Cell. 2013 Apr;24(7):982-94. doi: 10.1091/mbc.E12-09-0685. 
            Epub 2013 Feb 27.
  PUBMED    23447706
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E12-09-0685"; journalName: "Mol Biol Cell"; date: 
            "2013-04"; volume: "24"; issue: "7"; pages: "982-94"
COMMENT     Sequence Label: pEGFP-C1 Utr230-EGFP-3XNLS
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..71
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             75..95
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    270..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(307..326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     361..380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(535..556)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     747..766
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acgagctgta caagtccgga ctcagatctc gagctcaagc ttccatggat ccaaaaaaga
       61 agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaagtgat
      121 aatagggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      181 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      241 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      361 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
      421 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
      481 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
      541 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
      601 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
      661 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
      721 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
      781 ctggcaagtg tagcggtcac gctgcgcgt
//
