LOCUS       sequence-101784-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mRNA synthesis of Gal4ff codon modified for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2-Gal4ff.zf1
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             119..559
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    709..843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(733..752)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     787..806
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(956..976)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(956..974)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccgccaccat
      121 gaaactgctg agctccattg aacaggcctg tgatatctgc aggctgaaga agctgaagtg
      181 tagcaaggag aagcctaagt gtgctaagtg tctgaagaac aactgggagt gtagatacag
      241 ccctaagaca aagagaagcc ctctgacaag ggctcacctg acagaggtgg agagcagact
      301 ggagagactg gagcagctgt tcctgctgat cttccctaga gaggacctgg acatgatcct
      361 gaagatggac agcctccagg acatcaaggc tctgctgaca ggactgtttg tgcaggacaa
      421 tgtgaacaag gatgctgtga cagacagact ggcttctgtg gagacagaca tgcctctgac
      481 actgagacag cacagaatct ctgctacaag cagctctgag gagagcagca acaagggaca
      541 gagacagctg acagtgagcc ctgagttccc tgctgatgct ctggatgact ttgacctgga
      601 catgctgcct gctgatgctc tggatgactt tgacctggac atgctgcctg gaaactaaaa
      661 attcaaggcc tctcgagcct ctagaactat agtgagtcgt attacgtaga tccagacatg
      721 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      781 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      841 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      901 ttttaattcg cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt
      961 agtgagggtt aattgcgcgc ttggcgtaat catgg
//
