LOCUS       sequence-101788-        1124 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia 
            coli for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Zhang Y, Xie Q, Robertson JB, Johnson CH
  TITLE     pHlash: a new genetically encoded and ratiometric luminescence 
            sensor of intracellular pH.
  JOURNAL   PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Epub 
            2012 Aug 14.
  PUBMED    22905204
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(8):e43072. doi"; pages: " 
            10.1371/journal.pone.0043072. Epub 2012 Aug 14"
COMMENT     Sequence Label: pRSETb-pHlash
FEATURES             Location/Qualifiers
     source          1..1124
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..784)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     243..262
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     502..523
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(626..647)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agctctcttt cgggctttgt tagcagccgg atcaagcttc gaattcttac tcgatgttgt
       61 ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt
      121 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      181 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      241 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
      301 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact
      361 gcaggccgta gcccagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
      421 tgcagatcag cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
      481 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
      541 tgaacagctc ctcgcccttg ctcaccatgc caccgctgcc acccttgtac agctcgtcca
      601 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      661 cgttggggtc tttgctcagg gcggactggt agctcaggta gtggttgtcg ggcagcagca
      721 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt
      781 ccatgagctc ggcctgctcg ttcttcagca cgcgctccac gaagctcttg atgtacttac
      841 ccatttcatc tggagcgtcc tcctggagga agtggaggcc cttcaccttc acgaactcgg
      901 tgttagggaa cttcttagct ccctcgacaa tagcgttgga aaagaaccca gggtcggact
      961 cgatgaacag cttaggcaga tcgtcgctgg cccgaaggta ggcgttgtag ttgcggacaa
     1021 tctggacgac gtcgggcttg cctcccttaa cgagagggat ctcgcgaggc caggagaggg
     1081 taggccgtct aacctcgccc ttctccttga atggctccag gtag
//
