LOCUS       sequence-101790-         616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 616)
  TITLE     SGC Toronto Q2 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4TVR
FEATURES             Location/Qualifiers
     source          1..616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..40
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             53..73
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttaagaagg agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt
       61 gtatttccag ggcaatcagc catctacatc agctcgtgcc cgtcttattg atcctggctt
      121 tggaatatat aaggtaaatg aattggtgga tgccagagat gtcggccttg gtgcttggtt
      181 tgaagcacac atacatagtg ttactagagc ttctgatgga cagtcacgtg gcaaaactcc
      241 actgaagaat ggaaatataa agcataaatc caaagagaac acaaataaat tggacagtgt
      301 accctctacg tctaattcag actgtgttgc tgctgatgaa gacgttattt accatatcca
      361 gtatgatgaa tacccagaaa gcggtactct agaaatgaat gtcaaggatc ttagaccacg
      421 agctagaacc attttgaaat ggaatgaact aaatgttggt gatgtggtaa tggttaatta
      481 taatgtagaa agtcctggac aaagaggatt ctggtttgat gcagaaatta ccacattgaa
      541 gacaatctca aggaccaaaa aagaacttcg tgtgaaaatt ttcctggggg gttctgaagg
      601 aacattaaat gactgc
//
