LOCUS       sequence-101796-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of pHlash in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Zhang Y, Xie Q, Robertson JB, Johnson CH
  TITLE     pHlash: a new genetically encoded and ratiometric luminescence 
            sensor of intracellular pH.
  JOURNAL   PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Epub 
            2012 Aug 14.
  PUBMED    22905204
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(8):e43072. doi"; pages: " 
            10.1371/journal.pone.0043072. Epub 2012 Aug 14"
COMMENT     Sequence Label: pcDNA3.1+-pHlash
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             134..1066
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGAALAFHYAYEHQDRIKAIVHMESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETVLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc
      121 gagctcggat ccaatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
      181 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
      241 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctacctc
      301 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
      361 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
      421 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
      481 tgtgggccac gactgggggg ctgctctggc ctttcactac gcctacgagc accaagacag
      541 gatcaaggcc atcgtccata tggagagtgt cgtggacgtg atcgagtcct gggacgagtg
      601 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
      661 tgagaataac ttcttcgtcg agaccgtgct cccaagcaag atcatgcgga aactggagcc
      721 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
      781 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
      841 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaagc tgttcatcga
      901 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
      961 cgagttcgtg aaggtgaagg gcctccactt cctccaggag gacgctccag atgaaatggg
     1021 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcaggccg agctcatgga
     1081 cggcggc
//
