LOCUS       sequence-101797-        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of pHlash in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Zhang Y, Xie Q, Robertson JB, Johnson CH
  TITLE     pHlash: a new genetically encoded and ratiometric luminescence 
            sensor of intracellular pH.
  JOURNAL   PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Epub 
            2012 Aug 14.
  PUBMED    22905204
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(8):e43072. doi"; pages: " 
            10.1371/journal.pone.0043072. Epub 2012 Aug 14"
COMMENT     Sequence Label: pcDNA3.1+-pHlash
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..791)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     250..269
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     509..530
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(633..654)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgggccctc tagactcgag cggccgccac tgtgctggat atctgcagaa ttcttactcg
       61 atgttgtggc ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata
      121 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      181 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      241 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      301 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg
      361 aagcactgca ggccgtagcc cagggtggtc acgagggtgg gccagggcac gggcagcttg
      421 ccggtggtgc agatcagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      481 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      541 accccggtga acagctcctc gcccttgctc accatgccac cgctgccacc cttgtacagc
      601 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      661 cgcttctcgt tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc
      721 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
      781 ccgccgtcca tgagctcggc ctgctcgttc ttcagcacgc gctccacgaa gctcttgatg
      841 tacttaccca tttcatctgg agcgtcctcc tggaggaagt ggaggccctt caccttcacg
      901 aactcggtgt tagggaactt cttagctccc tcgacaatag cgttggaaaa gaacccaggg
      961 tcggactcga tgaacagctt aggcagatcg tcgctggccc gaaggtaggc gttgtagttg
     1021 cggacaatct ggacgacgtc gggcttgcct cccttaacga gagggatctc gcgaggccag
     1081 gagagggtag gccgtctaac ctcgcccttc tccttgaatg gctccaggta ggcagcga
//
