LOCUS       sequence-101799-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a single-chain TALEN targeting ABL1 site in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Sun N, Zhao H
  TITLE     A single-chain TALEN architecture for genome engineering.
  JOURNAL   Mol Biosyst. 2014 Mar 4;10(3):446-53. doi: 10.1039/c3mb70412b. Epub 
            2013 Dec 16.
  PUBMED    24336919
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70412b"; journalName: "Mol Biosyst"; date: "2014-03-4- 
            4"; volume: "10"; issue: "3"; pages: "446-53"
COMMENT     Sequence Label: pCMVJ-scTALEN-ABL1-R
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             67..87
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaattca gatctggtac cgccgccacc
       61 atgggaccta agaaaaagag gaaggtggcg gccgctgact acaaggatga cgacgataaa
      121 cttgatacat cgcttcttga ttcgatgcct gccgtcggca cgccgcatac agcggctgcc
      181 ccagcagagt gcgatgaggt gcaatcgggt ctgcgtgcag ccgatgaccc gccacccacc
      241 gtgcgtgtcg ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc gcgacggcgt
      301 gcggcgcaac cctccgacgc ttcgccggcc gcgcaggtgg atctacgcac gctcggctac
      361 agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac
      421 gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg
      481 gcagcgttag ggaccgttgc tgtcacgtat caggacataa tcagggcgtt gccagaggcg
      541 acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc tctggaggcc
      601 ttgctgaccg aagccggtga actgcgtggt ccgccgttgc agctggatac cggccaactg
      661 ttgaaaattg caaagcgtgg tggcgtgacc gcggtggaag cggtccatgc ctggcgtaat
      721 gcgttgacgg gcgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac
      781 ggtggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
      841 catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg
      901 ctcgaa
//
