LOCUS       sequence-101800-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTDH mutant Opt12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   McLachlan MJ, Johannes TW, Zhao H
  TITLE     Further improvement of phosphite dehydrogenase thermostability by 
            saturation mutagenesis.
  JOURNAL   Biotechnol Bioeng. 2008 Feb 1;99(2):268-74.
  PUBMED    17615560
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng."; date: "2008-02-1"; volume: "99(2)"; pages: "268-74"
COMMENT     Sequence Label: pET15b-Opt12
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatgct gccgaaactc gttataactc
      241 accgagtaca cgaagagatc ctgcaactgc tggcgccaca ttgcgagctg ataaccaacc
      301 agaccgacag cacgctgacg cgcgaggaaa ttctgcgccg ctgtcgcgat gctcaggcga
      361 tgatggcgtt catgcccgat cgggtcgatg cagactttct tcaagcctgc cctgagctgc
      421 gtgtaatcgg ctgcgcgctc aagggcttcg acaatttcga tgtggacgcc tgtactgccc
      481 gcggggtctg gctgaccttc gtgcctgatc tgttgacggt cccgactgcc gagctggcga
      541 tcggactggc ggtggggctg gggcggcatc tgcgggcagc agatgcgttc gtccgctctg
      601 gcaagttcaa gggctggcaa ccacatttct acggcacggg gctggataac gctacggtcg
      661 gcttccttgg catgggcgcc atcggactgg ccatggctga tcgcttgcag ggatggggcg
      721 cgaccctgca gtaccacgcg gcgaaggctc tggatacaca aaccgagcaa cggctcggcc
      781 tgcgccaggt ggcgtgcagc gaactcttcg ccagctcgga cttcatcctg ctggcgcttc
      841 ccttgaatgc cgataccctg catctggtca acgccgagct gcttgccctc gtacggccgg
      901 gcgctctgct tgtaaacccc tgtcgtggct cggtagtgga tgaagccgcc gtgctcgcgg
      961 cgcttgagcg aggccagctc ggcgggtatg cggcggatgt attc
//
