LOCUS       sequence-101802-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTDH mutant Opt13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   McLachlan MJ, Johannes TW, Zhao H
  TITLE     Further improvement of phosphite dehydrogenase thermostability by 
            saturation mutagenesis.
  JOURNAL   Biotechnol Bioeng. 2008 Feb 1;99(2):268-74.
  PUBMED    17615560
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng."; date: "2008-02-1"; volume: "99(2)"; pages: "268-74"
COMMENT     Sequence Label: pET15b-Opt13
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        65..83
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             164..181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             191..208
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
       61 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
      121 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
      181 cagcagcggc ctggtgccgc gcggcagcca tatgctgccg aaactcgtta taactcaccg
      241 agtacacgaa gagatcctgc aactgctggc gccacattgc gagctgataa ccaaccagac
      301 cgacagcacg ctgacgcgcg aggaaattct gcgccgctgt cgcgatgctc aggcgatgat
      361 ggcgttcatg cccgatcggg tcgatgcaga ctttcttcaa gcctgccctg agctgcgtgt
      421 aatcggctgc gcgctcaagg gcttcgacaa tttcgatgtg gacgcctgta ctgcccgcgg
      481 ggtctggctg accttcgtgc ctgatctgtt gacggtcccg actgccgagc tggcgatcgg
      541 actggcggtg gggctggggc ggcatctgcg ggcagcagat gcgttcgtcc gctctggcaa
      601 gttcaagggc tggcaaccac atttctacgg cacggggctg gataactcta cggtcggctt
      661 ccttggcatg ggcgccatcg gactggccat ggctgatcgc ttgcagggat ggggcgcgac
      721 cctgcagtac cacgcggcga aggctctgga tacacaaacc gagcaacggc tcggcctgcg
      781 ccaggtggcg tgcagcgaac tcttcgccag ctcggacttc atcctgctgg cgcttccctt
      841 gaatgccgat accctgcatc tggtcaacgc cgagctgctt gccctcgtac ggccgg
//
